Detection of Highly Prevalent Hepatitis B Virus Coinfection among HIV-Seropositive Persons in Ghana
Identifieur interne : 000A11 ( Main/Exploration ); précédent : 000A10; suivant : 000A12Detection of Highly Prevalent Hepatitis B Virus Coinfection among HIV-Seropositive Persons in Ghana
Auteurs : Anna Maria Geretti [Royaume-Uni] ; Mauli Patel [Royaume-Uni] ; Fred Stephen Sarfo [Ghana] ; David Chadwick [Royaume-Uni] ; Jens Verheyen [Allemagne] ; Maria Fraune [Allemagne] ; Ana Garcia [Royaume-Uni] ; Richard Odame Phillips [Ghana]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN viral (génétique), ADN viral (sang), Analyse de séquence d'ADN, Antigènes de surface du virus de l'hépatite B (génétique), Antigènes de surface du virus de l'hépatite B (sang), Charge virale, DNA-directed DNA polymerase (génétique), Ghana, Génotype, Humains, Hépatite B (diagnostic), Hépatite B (épidémiologie), Infections à VIH (), Mutation faux-sens, Protéines virales (génétique), Réaction de polymérisation en chaîne, Sensibilité et spécificité, Virus de l'hépatite B (), Virus de l'hépatite B (génétique), Virus de l'hépatite B (immunologie), Virus de l'hépatite B (isolement et purification).
- MESH :
- diagnostic : Hépatite B.
- génétique : ADN viral, Antigènes de surface du virus de l'hépatite B, DNA-directed DNA polymerase, Protéines virales, Virus de l'hépatite B.
- immunologie : Virus de l'hépatite B.
- isolement et purification : Virus de l'hépatite B.
- sang : ADN viral, Antigènes de surface du virus de l'hépatite B.
- épidémiologie : Hépatite B.
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- DNA, Viral (blood), DNA, Viral (genetics), DNA-Directed DNA Polymerase (genetics), Detection, Genotype, Ghana, HIV Infections (complications), Hepatitis B (diagnosis), Hepatitis B (epidemiology), Hepatitis B Surface Antigens (blood), Hepatitis B Surface Antigens (genetics), Hepatitis B virus, Hepatitis B virus (classification), Hepatitis B virus (genetics), Hepatitis B virus (immunology), Hepatitis B virus (isolation & purification), Human, Human immunodeficiency virus, Humans, Mixed infection, Mutation, Missense, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Viral Load, Viral Proteins (genetics).
- MESH :
- chemical , blood : DNA, Viral, Hepatitis B Surface Antigens.
- chemical , genetics : DNA, Viral, DNA-Directed DNA Polymerase, Hepatitis B Surface Antigens, Viral Proteins.
- geographic : Ghana.
- classification : Hepatitis B virus.
- complications : HIV Infections.
- diagnosis : Hepatitis B.
- epidemiology : Hepatitis B.
- genetics : Hepatitis B virus.
- immunology : Hepatitis B virus.
- isolation & purification : Hepatitis B virus.
- Genotype, Humans, Mutation, Missense, Polymerase Chain Reaction, Sensitivity and Specificity, Sequence Analysis, DNA, Viral Load.
Abstract
Simple hepatitis B surface antigen (HBsAg) tests may facilitate ascertainment of hepatitis B virus (HBV) infection in settings with high endemicity but limited infrastructure. We evaluated two rapid HBsAg tests and characterized HBV coinfection in a Ghanaian HIV-positive cohort. Samples from 838 patients were tested by the rapid assays Determine and Vikia and the reference assays Architect, Murex version 3, and Liaison Ultra. The assays were also evaluated using the 2nd International Standard, a seroconversion panel, and two mutant panels. HBsAg-positive samples underwent HBV DNA quantification by real-time PCR and surface and polymerase gene population sequencing. Overall, 140/838 patients (16.7%; 95% confidence interval, 14.2 to 19.2%) were HBsAg positive, and of these, 103/140 (73.6%) were e-antigen negative and 118/140 (84.3%) showed an HBV DNA level of > 14 IU/ml (median, 8,279 IU/ml). Assay sensitivities and specificities were as follows: Architect, 97.9 and 99.6%; Liaison, 97.1 and 99.4%; Murex, 98.6 and 99.3%; Determine, 69.3 and 100%; and Vikia, 70.7 and 100%. With Determine, the limit of detection was > 1.5 to 3.4 HBsAg IU/ml, and the median HBV DNA loads were 598 and 10,905 IU/ml in Determine-negative and -positive samples, respectively (P = 0.0005). Results were similar with the Vikia assay. HBV DNA sequencing indicated infection with genotype E in 82/86 (95.3%) patients. HBsAg mutations affected assay performance, including a T123A mutant that escaped detection by Architect. Major drug resistance mutations were observed in 4/86 patients (4.6%). The prevalence of HBV coinfection was high in this HIV-positive Ghanaian cohort. The two rapid assays identified HBsAg-positive patients at risk for liver disease with high specificity, albeit with only moderate sensitivity.
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i1="01"><s1>Department of Virology, Royal Free Hampstead NHS Trust and University College London Medical School</s1><s2>London</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Patel, Mauli" sort="Patel, Mauli" uniqKey="Patel M" first="Mauli" last="Patel">Mauli Patel</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Royal Free Hampstead NHS Trust and University College London Medical School</s1><s2>London</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Sarfo, Fred Stephen" sort="Sarfo, Fred Stephen" uniqKey="Sarfo F" first="Fred Stephen" last="Sarfo">Fred Stephen Sarfo</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Medicine, Komfo Anokye Teaching Hospital</s1><s2>Kumasi</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Ghana</country><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Chadwick, David" sort="Chadwick, David" uniqKey="Chadwick D" first="David" last="Chadwick">David Chadwick</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Infection & Travel Medicine, The James Cook University Hospital</s1><s2>Middlesbrough</s2><s3>GBR</s3><sZ>4 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Middlesbrough</wicri:noRegion></affiliation></author><author><name sortKey="Verheyen, Jens" sort="Verheyen, Jens" uniqKey="Verheyen J" first="Jens" last="Verheyen">Jens Verheyen</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute for Virology, University of Cologne</s1><s2>Cologne</s2><s3>DEU</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>University of Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Fraune, Maria" sort="Fraune, Maria" uniqKey="Fraune M" first="Maria" last="Fraune">Maria Fraune</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Institute for Virology, University of Cologne</s1><s2>Cologne</s2><s3>DEU</s3><sZ>5 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Allemagne</country><wicri:noRegion>Cologne</wicri:noRegion><wicri:noRegion>University of Cologne</wicri:noRegion><wicri:noRegion>Cologne</wicri:noRegion></affiliation></author><author><name sortKey="Garcia, Ana" sort="Garcia, Ana" uniqKey="Garcia A" first="Ana" last="Garcia">Ana Garcia</name><affiliation wicri:level="3"><inist:fA14 i1="01"><s1>Department of Virology, Royal Free Hampstead NHS Trust and University College London Medical School</s1><s2>London</s2><s3>GBR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author><author><name sortKey="Odame Phillips, Richard" sort="Odame Phillips, Richard" uniqKey="Odame Phillips R" first="Richard" last="Odame Phillips">Richard Odame Phillips</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Medicine, Komfo Anokye Teaching Hospital</s1><s2>Kumasi</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Ghana</country><wicri:noRegion>Kumasi</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="05"><s1>School of Medical Sciences, Kwame Nkrumah University of Science and Technology</s1><s2>Kumasi</s2><s3>GHA</s3><sZ>8 aut.</sZ></inist:fA14><country>Ghana</country><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of clinical microbiology : (Print)</title><title level="j" type="abbreviated">J. clin. microbiol. : (Print)</title><idno type="ISSN">0095-1137</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Viral (blood)</term><term>DNA, Viral (genetics)</term><term>DNA-Directed DNA Polymerase (genetics)</term><term>Detection</term><term>Genotype</term><term>Ghana</term><term>HIV Infections (complications)</term><term>Hepatitis B (diagnosis)</term><term>Hepatitis B (epidemiology)</term><term>Hepatitis B Surface Antigens (blood)</term><term>Hepatitis B Surface Antigens (genetics)</term><term>Hepatitis B virus</term><term>Hepatitis B virus (classification)</term><term>Hepatitis B virus (genetics)</term><term>Hepatitis B virus (immunology)</term><term>Hepatitis B virus (isolation & purification)</term><term>Human</term><term>Human immunodeficiency virus</term><term>Humans</term><term>Mixed infection</term><term>Mutation, Missense</term><term>Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Sequence Analysis, DNA</term><term>Viral Load</term><term>Viral Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>ADN viral (sang)</term><term>Analyse de séquence d'ADN</term><term>Antigènes de surface du virus de l'hépatite B (génétique)</term><term>Antigènes de surface du virus de l'hépatite B (sang)</term><term>Charge virale</term><term>DNA-directed DNA polymerase (génétique)</term><term>Ghana</term><term>Génotype</term><term>Humains</term><term>Hépatite B (diagnostic)</term><term>Hépatite B (épidémiologie)</term><term>Infections à VIH ()</term><term>Mutation faux-sens</term><term>Protéines virales (génétique)</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Virus de l'hépatite B ()</term><term>Virus de l'hépatite B (génétique)</term><term>Virus de l'hépatite B (immunologie)</term><term>Virus de l'hépatite B (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>DNA, Viral</term><term>Hepatitis B Surface Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term><term>DNA-Directed DNA Polymerase</term><term>Hepatitis B Surface Antigens</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Ghana</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Hepatitis B</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Hépatite B</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Hepatitis B</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Antigènes de surface du virus de l'hépatite B</term><term>DNA-directed DNA polymerase</term><term>Protéines virales</term><term>Virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Hepatitis B virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ADN viral</term><term>Antigènes de surface du virus de l'hépatite B</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Hépatite B</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genotype</term><term>Humans</term><term>Mutation, Missense</term><term>Polymerase Chain Reaction</term><term>Sensitivity and Specificity</term><term>Sequence Analysis, DNA</term><term>Viral Load</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Charge virale</term><term>Ghana</term><term>Génotype</term><term>Humains</term><term>Infections à VIH</term><term>Mutation faux-sens</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Virus de l'hépatite B</term><term>Virus hépatite B</term><term>Virus immunodéficience humaine</term><term>Homme</term><term>Détection</term><term>Infection mixte</term><term>Ghana</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Simple hepatitis B surface antigen (HBsAg) tests may facilitate ascertainment of hepatitis B virus (HBV) infection in settings with high endemicity but limited infrastructure. We evaluated two rapid HBsAg tests and characterized HBV coinfection in a Ghanaian HIV-positive cohort. Samples from 838 patients were tested by the rapid assays Determine and Vikia and the reference assays Architect, Murex version 3, and Liaison Ultra. The assays were also evaluated using the 2nd International Standard, a seroconversion panel, and two mutant panels. HBsAg-positive samples underwent HBV DNA quantification by real-time PCR and surface and polymerase gene population sequencing. Overall, 140/838 patients (16.7%; 95% confidence interval, 14.2 to 19.2%) were HBsAg positive, and of these, 103/140 (73.6%) were e-antigen negative and 118/140 (84.3%) showed an HBV DNA level of > 14 IU/ml (median, 8,279 IU/ml). Assay sensitivities and specificities were as follows: Architect, 97.9 and 99.6%; Liaison, 97.1 and 99.4%; Murex, 98.6 and 99.3%; Determine, 69.3 and 100%; and Vikia, 70.7 and 100%. With Determine, the limit of detection was > 1.5 to 3.4 HBsAg IU/ml, and the median HBV DNA loads were 598 and 10,905 IU/ml in Determine-negative and -positive samples, respectively (P = 0.0005). Results were similar with the Vikia assay. HBV DNA sequencing indicated infection with genotype E in 82/86 (95.3%) patients. HBsAg mutations affected assay performance, including a T123A mutant that escaped detection by Architect. Major drug resistance mutations were observed in 4/86 patients (4.6%). The prevalence of HBV coinfection was high in this HIV-positive Ghanaian cohort. The two rapid assays identified HBsAg-positive patients at risk for liver disease with high specificity, albeit with only moderate sensitivity.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Ghana</li><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Geretti, Anna Maria" sort="Geretti, Anna Maria" uniqKey="Geretti A" first="Anna Maria" last="Geretti">Anna Maria Geretti</name></region><name sortKey="Chadwick, David" sort="Chadwick, David" uniqKey="Chadwick D" first="David" last="Chadwick">David Chadwick</name><name sortKey="Garcia, Ana" sort="Garcia, Ana" uniqKey="Garcia A" first="Ana" last="Garcia">Ana Garcia</name><name sortKey="Patel, Mauli" sort="Patel, Mauli" uniqKey="Patel M" first="Mauli" last="Patel">Mauli Patel</name></country><country name="Ghana"><noRegion><name sortKey="Sarfo, Fred Stephen" sort="Sarfo, Fred Stephen" uniqKey="Sarfo F" first="Fred Stephen" last="Sarfo">Fred Stephen Sarfo</name></noRegion><name sortKey="Odame Phillips, Richard" sort="Odame Phillips, Richard" uniqKey="Odame Phillips R" first="Richard" last="Odame Phillips">Richard Odame Phillips</name><name sortKey="Odame Phillips, Richard" sort="Odame Phillips, Richard" uniqKey="Odame Phillips R" first="Richard" last="Odame Phillips">Richard Odame Phillips</name></country><country name="Allemagne"><noRegion><name sortKey="Verheyen, Jens" sort="Verheyen, Jens" uniqKey="Verheyen J" first="Jens" last="Verheyen">Jens Verheyen</name></noRegion><name sortKey="Fraune, Maria" sort="Fraune, Maria" uniqKey="Fraune M" first="Maria" last="Fraune">Maria Fraune</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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